Bonferroni’s method, not Tukey’s, should be used to control the total number of false positives when making multiple pairwise comparisons in experiments with few replicates. Zweifach A. SLAS Discov. 2025 Sep;35:100253. doi: 10.1016/j.slasd.2025.100253. Epub 2025 Jul 21. PMID: 40701522 Free article.

Determining how many cells to average for statistical testing of microscopy experiments. Zweifach A. J Cell Biol. 2024 Aug 5;223(8):e202401074. doi: 10.1083/jcb.202401074. Epub 2024 Jun 26. PMID: 38922298 Free PMC article.

Samples in many cell-based experiments are matched/paired but taking this into account does not always increase power of statistical tests for differences in means. Zweifach A. Mol Biol Cell. 2024 Jan 1;35(1):br1. doi: 10.1091/mbc.E23-05-0159. Epub 2023 Nov 1. PMID: 37910179 Free PMC article.